Desarrollo de interfaz opensmartflex-energis: módulos de peticiones y consumos

Pasantía (Ingeniero en Informática)-- Universidad Autónoma de occidente.2006PregradoIngeniero(a) en Informátic

Desarrollo de interfaz opensmartflex-energis: módulos de peticiones y consumos

Pasantía (Ingeniero en Informática)-- Universidad Autónoma de occidente.200

Sequential pressurized liquid extraction and subsequent supercritical antisolvent fractionation of mango seed kernel extracts with antiproliferative activity

Resumen del trabajo presentado al 17th European Meeting on Supercritical Fluids (EMSF) y al 7th European Meeting on High Pressure Technology, celebrados en Ciudad Real (España) del 8 al 11 de abril de 2019.[Introduction]: Mangifera indica L. (Anacardiaceae), commonly named mango, is a native plant to India and Myanmar and it is grown in tropical or subtropical regions. Mango is one of the main products for human consumption worldwide. The nutritional and functional value of the mango fruit constitute the fundamental reason for its wide diffusion as food. Mango is a good source of bioactive phytochemicals with antioxidant and antiproliferative activities. The mango seed kernel is one of the generated wastes during both the fresh consumption and the transformation process of the fruit. Depending on the varieties, kernel represents 45–85% of the seed and approximately 20% of the whole fruit3. Moreover, mango seed kernel is reported in literature as a potential source of bioactive compounds. Hence, considering the bioactive potential of this food byproduct, a revalorization strategy based on a sequential extraction and fractionation using compressed fluids was optimized in order to obtain polyphenolic-rich extracts from mango seed kernel with the highest antiproliferative activity against HT-29 colon cancer cells. In the first step, kernel was defatted evaluating different non-polar solvents (n-hexane, n-heptane, n-cyclohexane and (+)-limonene) under pressurized liquid extraction (PLE) conditions. In the second step, ethyl acetate and ethanol were used as extraction solvents at different temperatures to obtain polar extracts by an optimized PLE procedure. In the third and final step, the optimum extract was fractionated by supercritical antisolvent procedure (SAF). The optimization of the SAF process was carried out using a response surface methodology (RSM) by Box-Behnken design with three factors: CO2 pressure (80, 120 and 150 bar), percentage of water in the PLE extract (20, 30 and 50 % H2O v/v) and PLE extract/SC-CO2 flow ratio. The selected response was the antiproliferative activity against HT-29 colon cancer cells.[Results and discussion]: For the first step of the PLE procedure, n-heptane was selected due to the good performance exhibited in the extraction of the lipidic content of mango kernel seeds (12.88 %), compared to n-cyclohexane (8.15 %), (+)- limonene (2.35 %) and n-hexane (8.12 %). After the defatting process, the second step of the PLE procedure was optimized using a central composite design. For this purpose, solvent composition (percentage of EtOH in the mixture EtOH/EtOAc: 0, 50 and 100 % v/v) and temperature (50, 100 and 150 ºC) were evaluated to maximize extraction yield, total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity (EC50 and TEAC). Optimal extraction yield (12.43 %), TPC (138.37 mg GAE g-1), TFC (1.25 mg Quer g-1) and antioxidant activity (EC50 15.27 μg mL-1 and 2.14 mM trolox g-1) were obtained operating at 150 °C and 100 % EtOH v/v. The antiproliferative activity of the optimal PLE extract against HT-29 cells was also evaluated showing a 50 % of cell survival; the IC50 value of the extract was 28.67 μg mL-1 at 72 h of treatment. Then, the optimal PLE extract was fractionated by SAF process to obtain fractions with improved antiproliferative activity. By employing a RSM it was possible to optimize the most important factors involved in the SAF process. The fraction (extract) obtained operating at 150 bar, 50 % H20 v/v in the feeding solution and 0.0625 feed/SC-CO2 flow mass ratio presented the highest antiproliferative activity with 29.15 % of cell survival after 72 h of treatment considering the same IC50 concentration than the original PLE extract.[Conclusions]: In the present work, a sequential extraction and fractionation process based on the use of pressurized liquid extraction and supercritical antisolvent fractionation has been optimized to obtain mango seed kernel extracts with antiproliferative activity against HT-29 colon cancer cells. The SAF process allowed obtaining fractions with improved antiproliferative activity respect to the original PLE extract. Results show the high potential of this strategy for the valorization of the mango by-products.Peer reviewe

Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells

Mango seed kernel (MSK) has demonstrated to be a valuable source of bioactive phenolic compounds with antiproliferative activity against colon cancer cell lines. In this work, a phenolic MSK extract obtained by sequential pressurized liquid extraction (PLE) was submitted to supercritical antisolvent fractionation (SAF) to attain enriched fractions with improved inhibitory effect on HT-29 colon cancer cells. SAF was optimized using a Box-Behnken experimental design and considering as main factors: pressure, percentage of water in the feed extract and feed/sc−CO2 flow rate ratio. The highest inhibitory cell proliferation effect (70.51 ± 1.14%) was obtained in the extract using 50% of water v/v in the feed and 0.0625 feed/sc−CO2 ratio at 15 MPa. A targeted phytochemical profiling by LC-q-TOF-MS/MS followed by a multivariate statistical analysis of the observed bioactivity and the chemical composition evidenced the presence of characteristic compounds which might explain the enhanced antiproliferative activity of the optimal SAF extract.This research was supported by COOPA20145, project from CSIC (Programa de Cooperación Científica para el Desarrollo “i−COOP+”). G.A.-R. would like to acknowledge the Ministry of Economy and Competitiveness for a “Juan de la Cierva” postdoctoral grant. The authors also thank the support from the AGL2017-89417-R project.Peer reviewe

Análisis acústico de voz y de posturas labiales en pacientes de5 a 15 años con Labio y/o Paladar hendido corregido en la zona centro del país

IP 1119-14-1286

Research, Innovation and Extension to the service to society, in the framework of the Sixth Conference on Social Appropriation of Knowledge (SAK)

Annually, the Technological University of Pereira has been carrying out some events in the line of Social Appropriation of Knowledge. This has been done through the research, innovation, and extension Vice Rectory, moreover, these sessions are framed within the institutional objective: “Define and direct the guidelines for the institutional research that strengthen the research groups and the Seedbeds, through the formation of researchers, the development of science, technology, and innovation projects or programs, as well as the generation of networks and strategic partnerships that contribute to the creation and appropriation of knowledge for the society”. Therefore, the 6th Social Appropriation of Knowledge event took place under the title of “The research, Innovation, and Extension at the service of society” which was constituted as an academic and institutional opportunity where the results of the research projects from the last 5 years were published. The results of this event revealed, once again, the high academic level in investigation development at the university. There were 11 articles divided into 6 fields: Health, Engineering, Technology, Education, Industrial Technology, and Art, in which the results obtained by the research projects from the investigation groups were shown, promoting a knowledge exchange from their authors whose intellectual formation is diverse. With this publication, as part of a permanent effort to socialize the knowledge, the university promotes the circulation of its professors, students, and general community voices, having in mind that knowledge must be transferred through different channels, strengthening the academy and society in general, according to the institutional mission that invites us to incentivize a research culture in the university community.Presentation........................................................................................................... 5 Chapter 1. Health Teaching during the pandemic: what changes did professors implement? Results of a survey in a Colombian medical program. ........................................... 9 Germán Alberto Moreno Gómez ,Rodolfo Adrián Cabrales Vega, Jairo Franco Londoño, Samuel Eduardo Trujillo Henao, Víctor Manuel Patiño Suárez Evaluation of the effectiveness of a rat, rabbit and human intestine decellularization protocol...................................................................................... 19 Julio César Sánchez Naranjo, Laura Victoria Muñoz Rincón, Andrés Felipe Quiroz Ma zuera, Andrés Mauricio García Cuevas, Cristhian David Arroyave Durán, Fabián David Giraldo Castaño, Álvaro Guerra Solarte, Juliana Buitrago Jaramillo Exploration of the filtering functions of the intestine through a filtering loop model: an experimental approach towards a feasible renal replacement.............. 31 Julio César Sánchez Naranjo, Laura Victoria Muñoz Rincón, Andrés Mauricio García Cuevas, Álvaro Guerra Solarte y Juliana Buitrago Jaramillo Chapter 2. Engineering Identification of sociodemographic factors using multivariate analysis related to the dropout of Universidad Tecnológica de Pereira undergraduate students.... 47 Nelcy N Atehortua-Sanchez, Paula Marcela Herrera, Julian D Echeverry Correa Design and Construction of an HVDC-MMC Terminal on a Low Scale to Interconnection of Windfarms to the Electrical Grid........................................ 61 Diego Alberto Montoya Acevedo, Andrés Escobar Mejía Chapter 3. Technologies Preliminary study of cytototoxic and bactericidal activities of nonpolar extracts from seeds and peel of Persea americana cv Lorena ............................................ 85 Gloria Edith Guerrero Alvarez, Daniel Steven Fernández, Daniela Londoño Ramirez Cytototoxic and bactericidal activities of nonpolar extracts from seeds and peel of Persea americana cv Hass..................................................................................... 95 Gloria Edith Guerrero Alvarez, Gustavo Alfonso Cifuentes Colorado, Paula Daniela Sandoval Mossos Chapter 4. Education Pedro Henríquez Ureña traveler and Cosmopolitan ........................................... 107 William Marín Osorio Reading and writing in the training of our teachers: a commitment of all ......... 133 María Gladis Agudelo Gil, Gloria Inés Correa Aristizábal Chapter 5. Industrial engineering Tasks design to promote metacognitive regulation in discrete event simulation ......................................................................................................... 151 María Elena Bernal Loaiza, Manuela Gómez Suta, Rosario Iodice CONTENIDO Chapter 6. Arts The media feuilleton, between fiction and reality............................................... 169 Teresita Vásquez Ramíre

Biota Colombiana Volumen 18 No. 1 (2017)

Volumen 18 Número 1 de la revista Biota ColombianaBogotá, Colombi

Biodiversidad 2015. Estado y tendencias de la biodiversidad continental de Colombia

El propósito de este documento es fortalecer la capacidad de agentes públicos y privados para la aplicación de la PNGIBSE, que constituye en sí misma una apuesta de interfaz entre ciencia, política y sociedad en la perspectiva de construir sostenibilidad en el desarrollo. Además de ello, representa un insumo para el seguimiento a los compromisos del país frente a convenios e iniciativas internacionales (CDB, IPBES, OCDE), así como un mecanismo pedagógico para generar interés, conciencia y apropiación de las diferentes dimensiones de la biodiversidad del país.Bogotá, D. C., ColombiaInstituto de Investigación de Recursos Biológicos Alexander von Humboldtreporte.humboldt.org.coreporte.humboldt.org.co/biodiversidad/en

Biodiversidad 2017. Estado y tendencias de la biodiversidad continental de Colombia

En la cuarta versión del Reporte, que corresponde al año 2017, es una obligación preguntarnos cuál ha sido y es el papel de esta publicación y si ha abarcado la diversidad de formas y conceptos que definen el estado y el futuro de la biodiversidad colombiana. Las temáticas que constituyen la columna vertebral de cada uno de los reportes anuales responden a temas de pertinencia, nivel de incidencia y actualidad desde cada uno de los diferentes niveles de organización de la biodiversidad y buscan responder las siguientes preguntas fundamentales: 1) ¿Cómo se encuentra la biodiversidad del país? 2)¿Qué factores, en dónde y en qué medida está siendo afectada? 3)¿Cuáles son las iniciativas que desde la sociedad civil o a nivel de políticas públicas buscan evitar esa pérdida? 4)¿Cuáles son las grandes oportunidades para mejorar su gestión y manejo? Si bien evaluar la incidencia que puede tener el Reporte sobre acciones de gestión no es tarea fácil, se debe reconocer la buena acogida que han tenido los textos, las ilustraciones y la cifras entre los distintos tipos de lectores y el papel fundamental que ha jugado el Reporte en comunicar información de altísima calidad sobre la biodiversidad colombiana en diferentes momentos coyunturales. En ese sentido esta publicación es cada vez más una herramienta de consulta y referencia que está abierta al público tanto en formato impreso como digital, y de la misma manera busca fortalecerse para continuar brindando información relevante para la toma de decisiones en materia ambiental.BogotáSubdirección de Investigacione